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Title: Flavonoids by HPLC
Author(s): Bligh, Annie Sim Wan 
Author(s): Ogegbo, O.
Wang, Z.-T.
Issue Date: 2013
Publisher: Springer
Related Publication(s): Natural products: Phytochemistry, botany and metabolism of alkaloids, phenolics and terpenes
Start page: 2107
End page: 2144
Flavonoids are secondary plant metabolites that are synthesized via the shikimate pathway. HPLC has been an important tool for the separation of these metabolites in the last 4 decades. The coupling of HPLC with a number of detection technologies either online, in tandem, or off-line enables the identification of flavonoids in plant, food, and biological samples. This chapter provides an overview of flavonoid analysis by HPLC, including extraction of flavonoids in both aglycones and glycosides, separation by a selection of stationary and mobile phases, and finally, detection and identification by UV–VIS, fluorescence, electrochemical, mass spectrometry, and NMR spectroscopy.
DOI: 10.1007/978-3-642-22144-6_97
CIHE Affiliated Publication: No
Appears in Collections:HS Publication

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