Please use this identifier to cite or link to this item: https://repository.cihe.edu.hk/jspui/handle/cihe/3801
Title: Regional differences of tachykinin effects on smooth muscle and pacemaker potentials of the stomach, duodenum, ileum and colon of an emetic model, the house musk shrews
Author(s): Chan, Stella Sze Wa 
Lu, Zengbing 
Jiang, Bin 
Author(s): Liu, J. Y. H.
Deng, Y.
Hui, J. C. M.
Du, P.
Ng, H. S. H.
Yang, L.
Liu, L.
Khalid, A.
Ngan, M. P.
Cui, D.
Rudd, J. A.
Issue Date: 2023
Publisher: Elsevier
Journal: Neuropeptides 
Volume: 97
Abstract: 
Background and aims
The contractile effects of tachykinins on the gastrointestinal tract are well-known, but how they modulate slow-waves, particularly in species capable of emesis, remains largely unknown. We aimed to elucidate the effects of tachykinins on myoelectric and contractile activity of isolated gastrointestinal tissues of the Suncus murinus.

Methods
The effects of substance P (SP), neurokinin (NK)A, NKB and selective NK1 (CP122,721, CP99,994), NK2 (SR48,968, GR159,897) and NK3 (SB218,795, SB222,200) receptor antagonists on isolated stomach, duodenum, ileum and colon segments were studied. Mechanical contractile activity was recorded using isometric force displacement transducers. Electrical pacemaker activity was recorded using a microelectrode array.

Results
Compared with NKA, SP induced larger contractions in stomach tissue and smaller contractions in intestinal segments, where oscillation magnitudes increased in intestinal segments, but not the stomach. CP122,721 and GR159,897 inhibited electrical field stimulation-induced contractions of the stomach, ileum and colon. NKB and NK3 had minor effects on contractile activity. The inhibitory potencies of SP and NKA on the peristaltic frequency of the colon and ileum, respectively, were correlated with those on electrical pacemaker frequency. SP, NKA and NKB inhibited pacemaker activity of the duodenum and ileum, but increased that of the stomach and colon. SP elicited a dose-dependent contradictive pacemaker frequency response in the colon.

Conclusion
This study revealed distinct effects of tachykinins on the mechanical and electrical properties of the stomach and colon vs. the proximal intestine, providing a unique aspect on neuromuscular correlation in terms of the effects of tachykinin on peristaltic and pacemaker activity in gastrointestinal-related symptoms.
URI: https://repository.cihe.edu.hk/jspui/handle/cihe/3801
DOI: 10.1016/j.npep.2022.102300
CIHE Affiliated Publication: Yes
Appears in Collections:HS Publication

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