Simultaneous determination of six isoflavonoids in commercial Radix Astragali by HPLC-UV

Abstract

A HPLC-UV method for the quantification of six major isoflavonoids, calycosin 7-O-β-d-glucoside (<b>1</b>), formononetin 7-O-β-d-glucoside (<b>2</b>), (6αR, 11αR) 3-hydroxy-9,10-dimethoxypterocarpan-3-O-β-d-glucoside (<b>3</b>), 7,2′-dihydroxy-3′,4′-dimethoxyisoflavan-7-O-β-d-glucoside (<b>4</b>), calycosin (<b>5</b>) and formononetin (<b>6</b>), in Radix Astragali (<i>Huangqi</i>) was developed and validated. The method was proven to be sensitive, specific, accurate and precise, as well as effective and easy.