A new phenanthrene with a spirolactone from Dendrobium chrysanthum and its anti-inflammatory activities

Abstract

Investigation of phenolic patterns from the stems of <i>Dendrobium chrysanthum</i> by HPLC–PDA–MS has led to the isolation of a new phenanthrene derivative with a spirolactone ring, dendrochrysanene (<b>1</b>), that proved to suppress the mRNA level of TNF-α, IL8, IL10, and iNOS in murine peritoneal macrophages. The structure of 1 was characterized on the basis of various NMR (¹H, ¹³C, ¹H–¹H COSY, HMQC, and HMBC), mass spectrometry, and X-ray crystal diffraction data.