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Title: Characterization of two homogalacturonan pectins with immunomodulatory activity from green tea
Author(s): Bligh, Annie Sim Wan 
Author(s): Wang, H.
Wei, G.
Liu, F.
Banerjee, G.
Joshi, M.
Shi, S.
Lian, H.
Fan, H.
Gu, X.
Wang, S.
Issue Date: 2014
Publisher: MDPI
Journal: International Journal of Molecular Sciences 
Volume: 15
Issue: 6
Start page: 9963
End page: 9978
Two natural homogalacturonan (HG) pectins (MW ca. 20 kDa) were isolated from green tea based on their immunomodulatory activity. The crude tea polysaccharides (TPS1 and TPS2) were obtained from green tea leaves by hot water extraction and followed by 40% and 70% ethanol precipitation, respectively. Two homogenous water soluble polysaccharides (TPS1-2a and TPS1-2b) were obtained from TPS1 after purification with gel permeation, which gave a higher phagocytic effect than TPS2. A combination of composition, methylation and configuration analyses, as well as NMR (nuclear magnetic resonance) spectroscopy revealed that TPS1-2a and TPS1-2b were homogalacturonan (HG) pectins consisting of a backbone of 1,4-linked α-d-galacturonic acid (GalA) residues with 28.4% and 26.1% of carboxyl groups as methyl ester, respectively. The immunological assay results demonstrated that TPS1-2, which consisted mainly of HG pectins, showed phagocytosis-enhancing activity in HL-60 cells.
DOI: 10.3390/ijms15069963
CIHE Affiliated Publication: No
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