Please use this identifier to cite or link to this item: https://repository.cihe.edu.hk/jspui/handle/cihe/803
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dc.contributor.authorCheung, David Wing Shingen_US
dc.contributor.otherChan, B. C. L.-
dc.contributor.otherHan, X. Q.-
dc.contributor.otherLui, S. L.-
dc.contributor.otherWong, C. W.-
dc.contributor.otherWang, T. B. Y.-
dc.contributor.otherCheng, S. W.-
dc.contributor.otherIp, M.-
dc.contributor.otherHan, S. Q. B.-
dc.contributor.otherYang, X.-S.-
dc.contributor.otherJolivalt, C.-
dc.contributor.otherLau, C. B. S.-
dc.contributor.otherLeung, P. C.-
dc.contributor.otherFung, K. P.-
dc.date.accessioned2021-07-08T06:41:54Z-
dc.date.available2021-07-08T06:41:54Z-
dc.date.issued2015-
dc.identifier.urihttps://repository.cihe.edu.hk/jspui/handle/cihe/803-
dc.description.abstractObjectives The aims of this study were to identify the active ingredients from <i>Portulaca oleracea</i> L. (PO) that could provide synergism with antibiotics against methicillin-resistant <i>Staphylococcus aureus</i> (MRSA) and their possible mechanisms of resistance inhibition. Methods High-speed counter-current chromatography (HSCCC) coupled with gas chromatography-mass spectrometry and a panel of laboratory MRSA strains were used for checkerboard and efflux inhibitory assays. Key findings Linoleic and oleic acids were identified from HSCCC fraction 18 of PO with synergistic antibacterial activity when combined with erythromycin against RN4220/pUL5054. Ethidium bromide efflux inhibitory studies revealed that linoleic and oleic acids may interfere the activity of MsrA pump. By comparing among a panel of linoleic and oleic acids analogues, unsaturated fatty acids in salt form with cis configuration and an increase in number of double bonds were found to further increase the antibacterial activity when used alone or in combination with antibiotics. Conclusion This study reported for the first time that two active ingredients, namely linoleic and oleic acids, were identified from PO with synergistic antibacterial activity when combined with erythromycin against MRSA RN4220/pUL5054 and possibly act by inhibiting the efflux pumps of the bacteria cells.en_US
dc.language.isoenen_US
dc.publisherOxford University Pressen_US
dc.relation.ispartofJournal of Pharmacy and Pharmacologyen_US
dc.titleCombating against methicillin-resistant Staphylococcus aureus - two fatty acids from Purslane (Portulaca oleracea L.) exhibit synergistic effects with erythromycinen_US
dc.typejournal articleen_US
dc.identifier.doi10.1111/jphp.12315-
dc.contributor.affiliationSchool of Health Sciencesen_US
dc.relation.issn2042-7158en_US
dc.description.volume67en_US
dc.description.issue1en_US
dc.description.startpage107en_US
dc.description.endpage116en_US
dc.cihe.affiliatedNo-
item.grantfulltextopen-
item.fulltextWith Fulltext-
item.openairetypejournal article-
item.cerifentitytypePublications-
item.languageiso639-1en-
item.openairecristypehttp://purl.org/coar/resource_type/c_6501-
crisitem.author.deptSchool of Health Sciences-
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