Please use this identifier to cite or link to this item: https://repository.cihe.edu.hk/jspui/handle/cihe/1728
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dc.contributor.authorBligh, Annie Sim Wanen_US
dc.contributor.otherXu, Y.-
dc.contributor.otherZhang, Z. J.-
dc.contributor.otherGeng, F.-
dc.contributor.otherSu, S. B.-
dc.contributor.otherWhite, K. N.-
dc.contributor.otherBranford-White, C. J.-
dc.contributor.otherWang, Z. T.-
dc.date.accessioned2021-11-12T09:51:40Z-
dc.date.available2021-11-12T09:51:40Z-
dc.date.issued2010-
dc.identifier.urihttps://repository.cihe.edu.hk/jspui/handle/cihe/1728-
dc.description.abstractA Chinese herbal preparation, Qing'E formula (QEF), has been used clinically for treating osteoporosis in postmenopausal women by virtue of its kidney-invigorating function; however, no evidence base links QEF to estrogen replacement therapy. In this study, we undertake a characterization of estrogenic activity of QEF using an <i>in vivo</i> model of ovariectomized (OVX) mice together with <i>in vitro</i> studies with the MCF-7 cells for further molecular characterization. OVX mice were treated intragastrically with QEF at doses of 0.85, 1.7, and 3.4 g/kg per day for 4 weeks. QEF treatments restored the estrus cycle and demonstrated significant estrogenic activity, as indicated by reversal of uterine atrophy (six-fold increase in uterine weight), reduction in rectal temperature, and increased expression (1.6-fold) of estrogen receptors (ERs) in the uterus. Notably, the largest changes in these three parameters were found at the lowest dose. At the highest dose of QEF, significant changes were found in adrenal gland weight (30% increase), serum estradiol (E<sub>2</sub>) (60% increase), and luteinizing hormone (LH) (17% decrease) compared with untreated OVX controls. The data suggest estrogenic responses induced by QEF show tissue variation that reflects different affinities of ERs for QEF components. QEF could significantly induce luciferase expression (2.7-fold compared with control) from an estrogen response element luciferase reporter and induced expression of ERα and ERβ (1.2-fold and 1.7-fold respectively) in MCF-7 cells. Both activities were inhibited 80–90% by the estrogen antagonist ICI 182,780. This study demonstrates that QEF activity is mediated through estrogenic components and provides an evidence base for QEF treatment of postmenopausal symptoms.en_US
dc.language.isoenen_US
dc.publisherMary Ann Liebert, Inc.en_US
dc.relation.ispartofRejuvenation Researchen_US
dc.titleTreatment with Qing'E, a kidney-invigorating Chinese herbal formula, antagonizes the estrogen decline in ovariectomized miceen_US
dc.typejournal articleen_US
dc.identifier.doi10.1089/rej.2009.1000-
dc.contributor.affiliationSchool of Health Sciencesen_US
dc.relation.issn1557-8577en_US
dc.description.volume13en_US
dc.description.issue4en_US
dc.description.startpage479en_US
dc.description.endpage488en_US
dc.cihe.affiliatedNo-
item.fulltextWith Fulltext-
item.grantfulltextopen-
item.cerifentitytypePublications-
item.openairecristypehttp://purl.org/coar/resource_type/c_6501-
item.openairetypejournal article-
item.languageiso639-1en-
crisitem.author.deptSchool of Health Sciences-
crisitem.author.orcid0000-0002-4757-2159-
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