Please use this identifier to cite or link to this item: https://repository.cihe.edu.hk/jspui/handle/cihe/1713
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dc.contributor.authorBligh, Annie Sim Wanen_US
dc.contributor.otherHan, H.-
dc.contributor.otherYang, L.-
dc.contributor.otherXu. Y.-
dc.contributor.otherDing, Y.-
dc.contributor.otherZhang, T.-
dc.contributor.otherWang, Z.-
dc.date.accessioned2021-11-12T06:14:13Z-
dc.date.available2021-11-12T06:14:13Z-
dc.date.issued2011-
dc.identifier.urihttps://repository.cihe.edu.hk/jspui/handle/cihe/1713-
dc.description.abstractGeniposide, an iridoid glycoside, is an important and characteristic compound in the fruits of <i>Gardenia jasminoides</i> Ellis, a commonly used medicinal herb in Chinese traditional and folk medicine for the treatment of inflammation and jaundice. However, few studies have been carried out on the metabolism of geniposide. In this study, we have established a rapid and sensitive method using ultra-performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry (UPLC/ESI-QTOF-MS) for analysis of the metabolic profile of geniposide in rat urine after oral administration. A total of ten metabolites were detected and identified by comparing their fragmentation patterns with that of geniposide using Metabolynx™ and MassFragment™ software tools. The results revealed that the principal metabolism pathways of geniposide in rat occurred after deglycosylation of the irdoid glycoside take place and this is followed by glucuronidation and the pyran-ring cleavages. The major metabolite, the glucuronic acid conjugate of genipin as observed <i>in vivo</i>, was further confirmed by the <i>in vitro</i> enzymatic study. The results of this work have demonstrated the feasibility of the UPLC/ESI-QTOF-MS approach for rapid and reliable characterization of metabolites from iridoid compounds.en_US
dc.language.isoenen_US
dc.publisherWileyen_US
dc.relation.ispartofRapid Communications in Mass Spectrometryen_US
dc.titleIdentification of metabolites of geniposide in rat urine using ultra-performance liquid chromatography combined with electrospray ionization quadrupole time-of-flight tandem mass spectrometryen_US
dc.typejournal articleen_US
dc.identifier.doi10.1002/rcm.5216-
dc.contributor.affiliationSchool of Health Sciencesen_US
dc.relation.issn1097-0231en_US
dc.description.volume25en_US
dc.description.issue21en_US
dc.description.startpage3339en_US
dc.description.endpage3350en_US
dc.cihe.affiliatedNo-
item.fulltextWith Fulltext-
item.grantfulltextopen-
item.cerifentitytypePublications-
item.openairecristypehttp://purl.org/coar/resource_type/c_6501-
item.openairetypejournal article-
item.languageiso639-1en-
crisitem.author.deptSchool of Health Sciences-
crisitem.author.orcid0000-0002-4757-2159-
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